Clark School Home UMD

ISR Events Calendar

Event Information

ChBE Seminar Series: Prof. Peter Vekilov, U. of Houston
Tuesday, April 3, 2018
11:00 a.m.
2108 Chemical and Nuclear Engineering Building
For More Information:
Amy Karlsson
ajkarl@umd.edu
http://chbe.umd.edu/seminars/spring2018

Speaker: Peter Vekilov, Professor of Chemical and Biomolecular Engineering and Chemistry at the Univesrity of Houston

Title: Reversible fibrils of p53 nucleate within mesoscopic protein-rich clusters

Abstract:

The protein p53 is expressed in all cells and plays a central role in tumor suppression. Mutations in p53 are associated with about 50% of human cancers. Mutated p53 exhibits a dominant-negative (DN) activity, i.e., in the mixture of normal and mutated p53 proteins the anti-cancer activity of the wild-type species is inhibited. Furthermore, some of the mutants attain new functions, not present in the wild-type protein; this activity is referred to as gain of function (GoF) and it strongly contributes to cancer development. The mechanisms that define the DN and GoF activity of mutated p53 are not understood. We hypothesized that they may be intrinsically related with the aggregation propensity of wild-type and mutant p53 and explored the aggregation of the wild-type protein. We developed a procedure to express and purify p53 in milligram amounts by combined Ni-Sepharose and heparin sepharose chromatography. To detect and characterize p53 aggregates, we combined biophysical and molecular biology methods: oblique illumination microscopy, dynamic light scattering, sedimentation, spectroscopy, ThT and ANS fluorescence, and immunoblotting. We found that p53 aggregates at concentrations ca. 1000-fold lower than those observed with other proteins, but only at temperatures close to physiological and in crowded solutions. p53 forms both amyloid fibrils and mesoscopic clusters; clusters are preferred at low temperature, whereas fibrils dominate at near-physiological temperature. Aggregates of both classes are reversible, but the cluster dynamics are significantly faster, suggesting that the enhanced cluster formation might be a result of evolutionary pressure aimed at fast storage and release of this protein. Importantly, the nucleation of p53 fibrils is hosted by the protein-rich clusters. The ability to form amyloid fibrils may correlate with the DN and GoF activity of p53 in the transformation of normal cells to cancerous. Fibril nucleation  hosted by precursors opens novel pathways for fibril suppression by controlling the cluster properties.

This Event is For: Campus

Browse Events By Calendar

Calendar Home

« Previous Month    Next Month »

August 2018
SU M TU W TH F SA
1 2 3 4 w
5 6 7 8 9 10 11 w
12 13 14 15 16 17 18 w
19 20 21 22 23 24 25 w
26 27 28 29 30 31 w

Search Events


ISR lecture and seminar series

Distinguished Lecturer Series
Intelligent Automation Inc. Colloquia Series
Microsystems Seminar Series
Lockheed Martin Robotics Seminar Series
Advanced Networks Colloquia Series
Model-Based Systems Engineering Colloquia Series

Submit an event to the ISR calendar Click here

News links

Current news
Search news
News archives